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KMID : 0861420100140010090
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Korean Journal of Nuclear Medicine Technology
2010 Volume.14 No. 1 p.90 ~ p.93
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Analysis of Labelling Efficiency According to Differences of Rotating Time in a Asan Medical Center (AMC) RBC Labelling Method
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Jung Eun-Mi
Jung Woo-Young
Ryu Jae-Kwang
Shim Dong-Oh
Lee Yeong-Hee
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Abstract
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Purpose: In our nuclear medicine department, we suggested AMC RBC labeling method improved by modifying a part of existing modified in-vitro method to raise the efficiency of 99mTc-RBC labeling. However, it needs to be more additional time and efforts than existing modified in-vitro method because the AMC RBC labeling method has to carry out the centrifugal separation process for 3~5 minutes. Therefore, in this study, we conducted researches to aim to maintain stable labeling effects and supplement a problem about additional time by reducing rotating time when labeling 99mTc-RBC.
Materials and Methods: This research has been conducted the object of 30 patients who examined study using 99mTc-RBC and agreed to this research at our hospital from May 2009 to September 2009. We made 4 blood samples which consisted of ACD 1 cc along with 5 cc blood from each patient and used the AMC RBC labeling method. At this moment, each labeling efficiency was calculated by different rotating time 5 min, 10 min, 15 min, and 20 min and then we compared differences.
Results: As a result, When comparing the 99mTc-RBC labeling method efficiency by using the AMC RBC labeling method which differents from rotating time, each labeling efficiency were 92.3±5.0 % in 5 min, 95.9±5.0 % in 10 min, 97.4±4.9 % in 15 min and 97.7±4.8 % in 20 min. We analyzed differences of the labeling efficiency from change of rotating time by using an one-way ANOVA and verified that in Duncan method. There was relatively efficiency low in 5min rotating time and no statistically significant change in over.
Conclusions: When comparing a existing method, the AMC RBC labeling method which goes through the centrifugal
separation process again offers more favorable condition to combine RBC with 99mTc04 - by eliminating an plasma ingredient. When using the modified in-vitro method, we have almost 20 min to rotate to acquire stable labeling efficiency. But, when using the AMC RBC labeling method, we acquire labeling efficiency well what we want within only 10 min to rotate. Decrease of rotating time can complement the AMC RBC labeling method which goes through the centrifugal separation process again and also provide more rapid study such as G-Ibleeding study due to fast labeling.
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KEYWORD
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Rotating Time, 99mTc-RBC, AMC RBC Labelling method, Modified in-vitro method
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